課程資訊
課程名稱
植物分子生物學
PLANT MOLECULAR BIOLOGY 
開課學期
93-2 
授課對象
生物資源暨農學院  園產品處理及利用組  
授課教師
黃鵬林 
課號
HORT5014 
課程識別碼
628 U1190 
班次
 
學分
全/半年
半年 
必/選修
必修 
上課時間
星期五2,3,4(9:10~12:10) 
上課地點
4-106 
備註
處理組必選,二選一 須先修過基礎分子生物學或分子生物學或類似課程與杜宜殷合開 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/932pmb 
課程簡介影片
 
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課程概述

This course will focus on
the application of molecular
biology to plant physiology,
plant genetics, and plant
breeding.  

課程目標
The learner will gain the
basic capabilities to
understand and evaluate the
publications in the field of
plant molecular biology. 
課程要求
Plant physiology,
Biochemistry, and Basic
molecular biology 
預期每週課後學習時數
 
Office Hours
另約時間 
指定閱讀
 
參考書目
General

1. Alberts B, Bray D, Johnson A, Lewis J, Raff M, Roberts K & Walter P (1998)
Essential Cell Biology - An introduction to the molecular biology of the cell.
Garland Publishing Inc. New York & London.

2. Baxevanis AD & Ouellette BFF (2001) Bioinformatics – A Practical Guide to
the Analysis of Genes and Proteins, 2nd ed. John Wiley & Sons, Inc. 470pp.

3. Buchanan BB, Gruissem W & Jones RL (2000) Biochemistry and Molecular
Biology of Plants. American Society of Plant Physiologists, Rockville,
Maryland. 1367pp.

4. Cooper GM & Hausman RE (2004) The Cell: A Molecular Approach. 3rd ed. ASM
Press, Washington, DC, USA.713pp.

5. Dennis DT & Turpin DH (1990) Plant Physiology, Biochemistry and Molecular
Biology. Longman Scientific & Technical. 529pp.

6. Gibson G & Muse SV (2002) A Primer of Genome Science. Sinauer Associates
Inc. 347pp.

7. Grierson D & Covey SN (1988) Plant Molecular Biology, 2nd ed. Chapman and
Hall, New York. 233pp.

8. Glick BR & Pasternak JJ (2003) Molecular Biotechnology, 3rd ed. ASM Press,
Washington DC. 760pp.

9. Hannon GJ (2003) RNAi: A Guide to Gene Silencing. Cold Spring Harbor
Laboratory Press, Cold Spring Harbor, New York, USA. 435pp.

10. Hawes C & Satiat-Jeunemaitre B (2001) Plant Cell Biology, 2nd ed. Oxford
University Press, New York. 338pp.

11. Heldt HW (1997) Plant Biochemistry and Molecular Biology. Oxford
University Press, Oxford, New York & Tokyo. 522pp.

12. Howell SH (1998) Molecular Genetics of Plant Development. Cambridge
University Press, Cambridge. 365pp.

13. Hughes MA (1996) Plant Molecular Genetics. Addison Wesley Longman Limited.
Harlow. 236pp.

14. Hunt SP & Livesey FJ (2000) Functional Genomics. Oxford University Press,
New York. 253pp.

15. Lea PJ & Leegood RC (1999) Plant Biochemistry and Molecular Biology. John
Wiley & Sons, Inc., New York. USA. 364pp.

16. Lewin B (2004) Genes VIII. Pearson Prentice Hall, Pearson Education, Inc.
Upper Saddle River, NJ, USA. 1027pp.

17. Westhoff P (1998) Molecular Plant Development. Oxford University Press,
Oxford, New York & Tokyo. 272pp.

Lab manual

1. Draper J, Scott R, Armitage P & Walden R (1988) Plant Genetic
Transformation and Gene Expression - A Laboratory Manual. Blackwell Scientific
Publications, Oxford, London, Edinburgh, Boston, Palo Alto & Melbourne.

2. Gelvin SB & Schilperoort RA (1994) Plant Molecular Biology Manual, 2nd ed.
Kluwer Academic Publishers, Dordrecht, Boston, London.

3. Gilmartin PM & Bowler C (2002) Molecular Plant Biology Volume One – A
Practical Approach. Oxford University Press. 274pp.

4. Gilmartin PM & Bowler C (2002) Molecular Plant Biology Volume two – A
Practical Approach. Oxford University Press. 332pp.

5. Micklos DA & Freyer GA (2003) DNA Science: A First Course. 2nd ed. Cold
Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA. 575pp.

6. Sambrook J, Russell DW & Sambrook J (2001) Molecular Cloning: A Laboratory
Manual, 3rd ed. Vols 1, 2 & 3. Cold Spring Harbor Laboratory Press, New York.

7. Schuler MA & Zielinski RE (1989) Methods in Plant Molecular Biology.
Academic Press Inc, San Diego.

8. Shaw CH (1988) Plant Molecular Biology - A Practical Approach. IRL Press,
Oxford, Washington DC.

9. Weigel D & Glazebrook J (2002) Arabidopsis. A Laboratory Manual. Cold
Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 354pp.

10. Weissbach A & Weissbach H (1988) Methods for Plant Molecular Biology.
Academic Press Inc, San Diego.

11. Westermeier R & Naven T (2002) Proteomics in Practice – A Laboratory
Manual of Proteome Analysis. Wiley-VCH Verlag-GmbH, Weinheim. 316pp. 
評量方式
(僅供參考)
 
No.
項目
百分比
說明
1. 
期中考 
33% 
 
2. 
期末考 
33% 
 
3. 
報告 
34% 
 
 
課程進度
週次
日期
單元主題
第1週
2/25  Introduction (I) 
第2週
3/04  Introduction (II) 
第3週
3/11  Genome organization and chromatin structures 
第4週
3/18  Arabidopsis as a model plant for functional genomics 
第5週
3/25  Strategies of gene cloning 
第6週
4/01  Transposable elements and transposon tagging 
第7週
4/08  Structural analysis of plant genes 
第8週
4/15  Regulation of plant gene expression 
第9週
4/22  Molecular biology of chloroplasts and mitochondria in plants 
第10週
4/29  Midterm exam 
第11週
5/06  Signal perception and transduction (I) 
第12週
5/13  Signal perception and transduction (II) 
第13週
5/20  Flower development 
第14週
5/27  Sexual incompatibility, seed development, and germination 
第15週
6/03  Senescence and programmed cell death (I) 
第16週
6/10  Senescence and programmed cell death (II) 
第17週
6/17  Course review and discussion